New Biochemical Separations

3aOH 7aOH acetic acid acetone acetylated Acta adsorbent adsorption alkaloids alumina amino acids amount analysis applied aqueous bile acids Biochem Biophys cent Chem chloroform cholestane choline chroma Chromatog chromatogram chromatographic separation CO₂H CO₂Me colour components compounds containing counter-current distribution derivatives detection detector determined Developing solvent dextran dinitrophenyl eluted ether fatty acids filter paper formamide fraction Gas chromatographic gel filtration Gel G glass glycosides hydrolysis hydroxyapatite hydroxyl groups ionization layers lecithin methanol method methyl minutes mixture mobile phase molecular weight molecule neutral lipids obtained paper chromatography phosphatidyl ethanolamine phospholipids plate polar lipids prepared procedure proteins quantitative R-values reaction relative retention room temperature sample saturated scintillator sensitivity Sephadex serine shown in Fig Silica Gel silicic acid sodium soluble solution solvent solvent system spots Spray reagent stationary phase steroids sterol substances suitable Table technique thin-layer chromatography tion triglycerides triterpenoid tube unsaturated ОН