Plant Cryopreservation: A Practical Guide

Front Cover
Barbara B.M. Reed
Springer Science & Business Media, Oct 31, 2007 - Technology & Engineering - 514 pages

Plant Cryopreservation: A Practical Guide is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species.

 

Contents

CryopreservationPractical Considerations
3
2 Cryopreservation Theory 15
14
Development of PVSBased Vitrification and EncapsulationVitrification
33
References
51
Development of Encapsulation Dehydration
59
Cryopreservation of Algae
95
Cryopreservation of Dedifferentiated Cell Cultures
141
Maurizio Lambardi E Aylin Ozudogru and Carla Benelli
166
through Air Desiccation
230
Bart Panis
241
Yanhua Zhao Yongjie Wu Yongjian Chang and Barbara M Reed
342
Cryopreservation of In Vitro Tissues of Deciduous Forest Trees
365
S Ganeshan P E Rajasekharan S Shashikumar and William Decruze
394
Modified PVS2
406
Cryopreservation of Dormant Buds
421
Cryopreservation of Pollen
443

References
188
Cryopreservation of Excised Embryos and Embryonic Axes
211
References
217

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