Plant Cryopreservation: A Practical GuideBarbara B.M. Reed Plant Cryopreservation: A Practical Guide is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species. |
Contents
3 | |
2 Cryopreservation Theory 15 | 14 |
Development of PVSBased Vitrification and EncapsulationVitrification | 33 |
References | 51 |
Development of Encapsulation Dehydration | 59 |
Cryopreservation of Algae | 95 |
Cryopreservation of Dedifferentiated Cell Cultures | 141 |
Maurizio Lambardi E Aylin Ozudogru and Carla Benelli | 166 |
through Air Desiccation | 230 |
Bart Panis | 241 |
Yanhua Zhao Yongjie Wu Yongjian Chang and Barbara M Reed | 342 |
Cryopreservation of In Vitro Tissues of Deciduous Forest Trees | 365 |
S Ganeshan P E Rajasekharan S Shashikumar and William Decruze | 394 |
Modified PVS2 | 406 |
Cryopreservation of Dormant Buds | 421 |
Cryopreservation of Pollen | 443 |