Recombinant DNA Technology, Volume 1

15 minutes 30 minutes acetate agar agarose gel aliquots ampicillin antibodies antigen autoradiography bacteria bacteriophage blotting buffer cDNA cells Centrifuge chloride cloning coli colony concentration containing Cosmid culture digestion dilution distilled water DNA fragments DNA ligase DNA molecules DNA polymerase ECORI EDTA elute Eppendorf tube ethanol eukaryotic extract filter paper foreign DNA gel electrophoresis gene genome H₂O hybrid hybridisation Incubate at 37°C inserted Isolation kinase labelled lambda ligation linkers litre medium mg/ml MgCl2 microlitres mixture mRNA NaCl nitrocellulose nitrocellulose filter nuclease nucleotide overnight parasites pellet phage phenol phosphate plaques plasmid plate Preparation probe procedure protein Protocol reaction recombinant DNA Remove restriction endonucleases restriction enzyme Resuspend room temperature sample screen sequence serum sodium Steps sterile stock solution Store at 20°C strain supernatant synthesis Transfer Tris Tris-Cl vector volume Wash µg/ml