Recombinant DNA Technology, Volume 1

30 minutes acetate acid activity agar agarose gel aliquots Allow antibodies antigen bacteria bacteriophage base blotting buffer Caesium chloride cDNA cells Centrifuge chloride cloning coli colony column complete concentration containing culture digestion dilution Dissolve distilled water DNA fragments ECORI EDTA electrophoresis ethanol expression extract filter gene genetic genome glass grow Heat hybrid hybridisation Incubate inserted Isolation labelled ligation loading Materials medium method mg/ml minutes mixture molecules mRNA NaCl nitrocellulose nucleotide obtained overnight parasites pellet phage phosphate Place plasmid plate Preparation presence probe procedure production protein reaction recombinant DNA Remove restriction enzyme Resuspend room temperature sample screen sequence single sodium solution specific Steps Store strain stranded supernatant synthesis Transfer Tris tube Type units vector volume Wash Western blotting