GB 5009.212-2016 Translated English of Chinese Standard. GB 5009.212-2016: National Food Safety Standard - Determination of Diarrheic Shellfish Poison in Shellfishhttps://www.chinesestandard.net, 2019 M06 18 - 22 pages This Standard specifies mouse biology method, enzyme linked immunosorbent assay (ELISA method) and liquid chromatography - tandem mass spectrometry for the determination of diarrheic shellfish poison in shellfish. In this Standard, mouse biology method and enzyme linked immunosorbent assay (ELISA method) are applicable to the determination of diarrheic shellfish poison in shellfish and shellfish products; liquid chromatography - tandem mass spectrometry is applicable to the determination of diarrheic shellfish poison okadaic acid (OA), dinophysistoxins-1 (DTX-1) and dinophysistoxins-2 (DTX-2) in edible part of shellfish and shellfish products (excluding salted products). |
Contents
Liquid Chromatography Tandem Mass Spectrometry | 12 |
Instruments and Equipment | 14 |
Expression of Analytical Result | 18 |
Precision | 19 |
Appendix A Evaluation Technical Parameters of Commercial Kit | 20 |
Appendix B Virulence Factor of Diarrheic Shellfish Poison | 21 |
Multiple Reaction Monitoring Chromatogram of Diarrheic Shellfish Poison Standard Solution | 22 |
Common terms and phrases
accordance with Formula accordance with Table accurately weigh-take acetone acetonitrile ammonium formate anhydrous ether assay ELISA method chromatographic purity concentration of diarrheic constant volume cross reaction rate Determination of Diarrheic diarrheic shellfish poison Diluent Dinophysistoxins-2 DTX-2 Disodium hydrogen phosphate enzyme linked immunosorbent enzyme marker Homogenize Hydrochloric acid immunosorbent assay ELISA linked immunosorbent assay liquid chromatography mass concentration measure-take Methanol solution 90 mL of methanol mL of water Mobile phase Mouse Biology Method ng/mL Okadaic acid OA open the shell PBS solution Poison in Shellfish poison standard solution Potassium dihydrogen phosphate quantitation limit r/min reach a constant rinse room temperature round-bottomed flask Sample Extraction sample solution series working solution shell meat shellfish poison standard SN/T Sodium hydroxide solid phase extraction solution 2.5 mol/L solvent control group standard substance standard working solution Sulfuric acid tandem mass spectrometry test group test stock solution Tween-60 saline solution various diarrheic shellfish Virulence Factor water to dilute