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180 pg ampicillin 7.5 mg/ml PRO-FAM Atlantic Salmon Bicine bovine serum albumin control P 0.05 cryopreservation trials cryoprotectants days post days post-fertilization DMSO egg lot eggs fertilized Esox ethylene glycol evaluated extender mixtures extender with I5 fertilize eggs fertilized with fresh fresh semen control frozen semen g glucose/2 liters g mannitol/2 liters glucose/2 liters extender glucose/liter extender I5 g glucose/2 less than fresh lots of eggs male walleye mannitol Mean of Pooled mg/ml BSA mixed with extender mixtures at Spirit ml 8 oz motility muskellunge eggs muskellunge semen Percent viability pg/ml ampicillin post-fertilization during cryopreservation production scale trials rainbow trout Rathbun Fish Hatchery refrigerated semen semen extender semen frozen semen mixed Semen preserved Semen Table Signiﬁcantly less sperm cells Spirit Lake Fish Spirit Lake Hatchery Stoss and Holtz tests trials at Rathbun trials with semen viability of muskellunge viability of walleye walleye and muskellunge walleye eggs walleye extender walleye semen