PCR Methods and Applications, Volume 4, Issues 1-3Cold Spring Harbor Laboratory Press, 1994 - Gene amplificaiton |
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Common terms and phrases
agarose gel AGLCR aliquot allele Alu repeats amplification analysis annealing antibody assay bands buffer cassette cell lines cloning coefficients Cold Spring Harbor containing copy number cycles denatured detection dideoxy digestion diluted DNA fragments DNA polymerase DNA sequence dNTP efficiency electrophoresis enzyme ethidium bromide exon extracted FIGURE fingerprinting first-strand GeneAmp genetic genomic genomic DNA human hybridization incubated isolated lane ligation markers merase MgCl2 molecular mRNA mutations Natl Nucleic Acids Nucleic Acids Res nucleotides oligomer oligonucleotide optimal PCR amplification PCR products PCR reaction performed Perkin-Elmer phage phagemid plasmid polyacrylamide gel polymerase chain reaction polymorphism pool primer probes procedure protein protocol pseudogenes quantitative quence RAPD reagents region replicate restriction reverse transcription ribozyme RT-PCR screening segment sensitivity signal single-stranded Southern blot specific Spring Harbor Laboratory SSCP SSCP component strand Taq polymerase target template tion tissue transfected tube vector vectorette vitro