Technical Bulletin, Issue 6

1.5 ml semen 125 egg sample 175 ml lake 3.0 ml extended 60 Sec Add 60 seconds 8-ounce egg lots activated with 175 addition of semen control egg lots control lots dry method egg depth egg group Egg Height Egg Lots Test eggs incubated eggs per 125 extended semen extended walleye semen Fish Culture Research fuller's earth hatching jar included multiple semen initial sperm cell initially mixed live eggs live walleye eggs lots 30 minute fertilization period mixed with 1.5 ml extended walleye ml lake water mm egg depth multiple addition multiple semen addition Number of live ounce egg Percent survival Rathbun Fish Culture sample when treatments seconds after initial Semen Added semen and sperm semen to extender semen was added significantly different sperm cell activation sperm cell motility survival of walleye T₁ T₂ Table Test 1 Test test egg lots test lots urea walleye eggs sampled water hardening